1: Biochim Biophys Acta  1981 Jan 14;634(1):85-92 

Nanosecond time-resolved fluorescence investigations of temperature-induced
conformational changes in cytochrome oxidase in phosphatidylcholine vesicles and
solubilized systems.

Kawato S, Yoshida S, Orii Y, Ikegami A, Kinosita K Jr.

Intrinsic and lipid phase transition-induced conformational changes in
cytochrome oxidase in phosphatidylcholine vesicle and solubilized systems were
examined by the fluorescence lifetime of N-(1-anilinonaphthyl-4)-maleimide
conjugated with the enzyme. The time-dependent fluorescence intensity of
N-(1-anilinonaphthyl-4)-maleimide attached to cytochrome oxidase was described
as a triple exponential decay. Both the intrinsic and lipid phase
transition-induced conformational changes were detectable in plots of the
average lifetime against temperature. In most cases a peak occurred at the
temperature of the conformational change. The time-dependent emission anisotropy
showed that N-(1-anilinonaphthyl-4)-maleimide embedded in cytochrome oxidase in
phosphatidylcholine vesicles underwent a rapid restricted wobbling within a
cone. The half-angle of the cone was around 30 degrees for cytochrome oxidase in
dimyristoyl phosphatidylcholine vesicles.

PMID: 6258646 [PubMed - indexed for MEDLINE]