1: J Biochem (Tokyo)  1983 Jun;93(6):1705-7 

Internal motion of F-actin in 10(-6)-10(-3) s time range studied by transient
absorption anisotropy: detection of torsional motion.

Mihashi K, Yoshimura H, Nishio T, Ikegami A, Kinosita K Jr.

By means of laser flash photolysis, the transient absorption anisotropy (TAA) of
the triplet probe, 5-iodoacetamide-Eosin, labeling rabbit skeletal F-actin was
measured in the 10(-6)-10(-3) s time range. The TAA curve at 20 degrees C showed
a relatively slow decay phase covering several hundred microseconds and a large
residual anisotropy (approximately 0.1 at 2 ms). After analysis with Barkley &
Zimm's formula, it was concluded that the TAA of Eosin-F-actin can be
approximated by the anisotropy decay due to torsional motion of F-actin.

PMID: 6885746 [PubMed - indexed for MEDLINE]