1: Biochim Biophys Acta  1984 Sep 5;775(3):374-80 

The dynamics of lipid motion in sarcoplasmic reticulum membranes determined by
steady-state and time-resolved fluorescence measurements on
1,6-diphenyl-1,3,5-hexatriene and related molecules.

Stubbs CD, Kinosita K Jr, Munkonge F, Quinn PJ, Ikegami A.

Steady-state and time-resolved fluorescence anisotropy measurements were made on
1,6-diphenyl-1,3,5-hexatriene (DPH),
1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene (TMA-DPH) and
1-acyl-2-(DPH)-phosphatidylcholine (DPH-PC) incorporated into sarcoplasmic
reticulum membranes. The results were analysed in terms of the
'wobbling-in-cone' model. Considerable differences in the fluorescence
parameters were found. In particular TMA-DPH and DPH-PC showed a smaller cone
angle, relating to the range of acyl chain motion, compared to DPH, taken to be
a reflection of a difference in probe locations. The influence of the protein
component was also found to restrict DPH motion more than TMA-DPH and DPH-PC.
Effectiveness in assessment of perturbation of the membrane by the
non-esterified fatty acid, oleic acid again revealed differences. The
steady-state anisotropy decreased on addition of oleic acid; a recovery to
control values was observed with DPH but not with the other probes.
Time-resolved parameters followed the same pattern. The results of this work
demonstrated the effectiveness of these three probes in revealing differences in
membrane properties, such as protein and fatty acid perturbation of membrane
lipid structure and dynamics.

PMID: 6466678 [PubMed - indexed for MEDLINE]