1: J Cell Biol  1991 Oct;115(1):67-73 

Dual-view microscopy with a single camera: real-time imaging of molecular
orientations and calcium.

Kinosita K Jr, Itoh H, Ishiwata S, Hirano K, Nishizaka T, Hayakawa T.

Department of Physics, Faculty of Science and Technology, Keio University,
Yokohama, Japan.

A new microscope technique, termed "W" (double view video) microscopy, enables
simultaneous observation of two different images of an object through a single
video camera or by eye. The image pair may, for example, be transmission and
fluorescence, fluorescence at different wavelengths, or mutually perpendicular
components of polarized fluorescence. Any video microscope can be converted into
a dual imager by simple insertion of a small optical device. The continuous
appearance of the dual image assures the best time resolution in existing and
future video microscopes. As an application, orientations of actin protomers in
individual, moving actin filaments have been imaged at the video rate.
Asymmetric calcium influxes into a cell exposed to an intense electric pulse
have also been visualized.

PMID: 1918140 [PubMed - indexed for MEDLINE]