1: Biophys J  1995 Aug;69(2):323-8 

Real time imaging of single fluorophores on moving actin with an epifluorescence
microscope.

Sase I, Miyata H, Corrie JE, Craik JS, Kinosita K Jr.

Department of Physics, Faculty of Science and Technology, Keio University,
Yokohama, Japan.

Relatively simple modifications of an ordinary epifluorescence microscope have
greatly reduced its background luminescence, allowing continuous and real time
imaging of single fluorophores in an aqueous medium. Main modifications were
changing the excitation light path and setting an aperture stop so that stray
light does not scatter inside the microscope. A simple and accurate method using
actin filaments is presented to establish the singularity of the observed
fluorophores. It was possible, at the video rate of 30 frames/s, to image
individual tetramethylrhodamine fluorophores bound to actin filaments sliding
over heavy meromyosin. The successful imaging of moving fluorophores
demonstrates that conventional microscopes may become a routine tool for
studying dynamic interactions among individual biomolecules in physiological
environments.

PMID: 8527645 [PubMed - indexed for MEDLINE]