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Synthesis of fluorescent substrates for protein tyrosine phosphatase assays.
Watanabe T, Imoto M, Taniguchi H, Kinoshita K Jr, Umezawa K.
Institute of Microbial Chemistry, Tokyo, Japan.
Two fluorescent substrates for protein tyrosine phosphatase (PTPase) reaction were prepared by conjugation of commercially available O-phosphotyrosine and dansyl chlorides. They were hydrolyzed by CD45 tyrosine phosphatase, and proved to be useful for PTPase assay.
PMID: 9871754 [PubMed - indexed for MEDLINE]
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