Home

Members

Research

Publications

Movies

Link

Original papers

Reviews

Reviews in Japanese

LIST of ORIGINAL PAPERS

2015

"Simple mechanism whereby the F₁-ATPase motor rotates with near-perfect chemomechanical energy conversion"
Ei-ichiro Saita, Toshiharu Suzuki, Kazuhiko Kinosita Jr., and Masasuke Yoshida
Proc. Natl. Acad. Sci. USA 112 (2015) 9626-9631. Abstract PDF (1394 kB) Journal Supporting Information (1719 kB) combined PDF (3031 kB)

"Direct observation of DNA overwinding by reverse gyrase"
Taisaku Ogawa, Katsunori Yogo, Shou Furuike, Kazuo Sutoh, Akihiko Kikuchi, and Kazuhiko Kinosita, Jr.
Proc. Natl. Acad. Sci. USA 112 (2015) 7495-7500. Abstract PDF (1515 kB) Journal Supporting Information (1166 kB) combined PDF (2651 kB) Movie (3076 kB)

2014

"None of the rotor residues of F₁-ATPase are essential for torque generation"
Ryohei Chiwata, Ayako Kohori, Tomonari Kawakami, Katsuyuki Shiroguchi, Shou Furuike, Kengo Adachi, Kazuo Sutoh, Masasuke Yoshida, and Kazuhiko Kinosita, Jr.
Biophys. J. 106 (2014) 2166-2174. Abstract PDF (2349 kB) Journal

2013

"Accurate polarity control and parallel alignment of actin filaments for myosin-powered transport systems"
Makito Miyazaki, Kazuhiko Kinosita Jr, and Katsuyuki Shiroguchi
RSC Advances 3 (2013) 8728-8733. Abstract PDF (1804 kB) Journal Supporting Information (112 kB) Movie (1899 kB)

2012

"Controlled rotation of the F₁-ATPase reveals differential and continuous binding changes for ATP synthesis"
Kengo Adachi, Kazuhiro Oiwa, Masasuke Yoshida, Takayuki Nishizaka, and Kazuhiko Kinosita Jr.
Nat. Commun. 3 (2012) 1022.     Abstract     PDF (6724 kB)     Journal     Supplementary Information (2074 kB)

"Direct observation of strand passage by DNA-topoisomerase and its limited processivity"
Katsunori Yogo, Taisaku Ogawa, Masahito Hayashi, Yoshie Harada, Takayuki Nishizaka, and Kazuhiko Kinosita Jr.
PLoS ONE 7 (2012) e34920.     Abstract     PDF (384 kB)     Journal     Supporting Information (original) SI (combined, 1798 kB)    Movie (692 kB)

"Kinetic equivalence of transmembrane pH and electrical potential differences in ATP synthesis"
Naoki Soga, Kazuhiko Kinosita, Jr., Masasuke Yoshida and Toshiharu Suzuki
J. Biol. Chem. 287 (2012) 9633–9639.     Abstract     PDF (971 kB)     Journal     Supplemental Data (852 kB)

"Torque generation and utilization in the motor enzyme F_oF₁-ATP synthase: half-torque F₁ with short-sized pushrod helix and reduced ATP synthesis by half-torque F_oF₁"
Eiji Usukura, Toshiharu Suzuki, Shou Furuike, Naoki Soga, Ei-ichiro Saita, Toru Hisabori, Kazuhiko Kinosita Jr., and Masasuke Yoshida
J. Biol. Chem. 287 (2012) 1884–1891.     Abstract     PDF (2316 kB)     Journal

2011

"Efficient ATP synthesis by thermophilic Bacillus F_oF₁-ATP synthase"
Naoki Soga, Kazuhiko Kinosita Jr., Masasuke Yoshida, and Toshiharu Suzuki
FEBS J. 278 (2011) 2647–2654.     Abstract     PDF (397 kB)     Journal

"Torque generation in F₁-ATPase devoid of the entire amino-terminal helix of the rotor that fills half of the stator orifice"
Ayako Kohori, Ryohei Chiwata, Mohammad Delawar Hossain, Shou Furuike, Katsuyuki Shiroguchi, Kengo Adachi, Masasuke Yoshida, and Kazuhiko Kinosita Jr.
Biophys. J. 101 (2011) 188–195.     Abstract     PDF (1369 kB)     Journal     Supporting material (18.8 kB)

"Direct observation of the myosin Va recovery stroke that contributes to unidirectional stepping along actin"
Katsuyuki Shiroguchi, Harvey F. Chin, Diane E. Hannemann, Eiro Muneyuki, Enrique M. De La Cruz, and Kazuhiko Kinosita Jr.
PLoS. Biol. 9 (2011) e1001031.     Abstract     PDF (2363 kB)     Journal     Supplemental Information (614 kB)     Movie (1486 kB)

"Resolving stepping rotation in Thermus thermophilus H⁺-ATPase/synthase with an essentially drag-free probe"
Shou Furuike, Masahiro Nakano, Kengo Adachi, Hiroyuki Noji, Kazuhiko Kinosita Jr., and Ken Yokoyama
Nat. Commun. 2 (2011) 233.     Abstract     PDF (3750 kB)     Journal     Supplemental material (3249 kB)

2010

"Activation and stiffness of the inhibited states of F₁-ATPase probed by single-molecule manipulation"
Ei-ichiro Saita, Ryota Iino, Toshiharu Suzuki, Boris A. Feniouk, Kazuhiko Kinosita, Jr., and Masasuke Yoshida
J. Biol. Chem. 285 (2010) 11411–11417.     Abstract     PDF (1348 kB)     Journal      Supplemental Data (1326 kB)

"Chemo-mechanical coupling in F₁-ATPase revealed by catalytic site occupancy during catalysis"
Rieko Shimo-Kon, Eiro Muneyuki, Hiroshi Sakai, Kengo Adachi, Masasuke Yoshida, and Kazuhiko Kinosita Jr.
Biophys. J. 98 (2010) 1227–1236.     Abstract     PDF (1943 kB)     Journal     Supporting material (420 kB)

"Stimulation of F₁-ATPase activity by sodium dodecyl sulfate"
Mohammad Delawar Hossain, Shou Furuike, Yasuhiro Onoue, Kengo Adachi, Masasuke Yoshida, and Kazuhiko Kinosita Jr.
Biochim. Biophys. Acta (Bioenergetics) 1797 (2010) 435-442.     Abstract     PDF (1762 kB)     Journal

2009

"A giant liposome for single-molecule observation of conformational changes in membrane proteins"
Yasuhiro Onoue, Toshiharu Suzuki, Max Davidson, Mattias Karlsson, Owe Orwar, Masasuke Yoshida, and Kazuhiko Kinosita Jr.
Biochim. Biophys. Acta (Biomembranes) 1788 (2009) 1332-1340. Abstract PDF (1277 kB) Journal

"Effect of ε subunit on the rotation of thermophilic Bacillus F₁-ATPase"
Masato Tsumuraya, Shou Furuike, Kengo Adachi, Kazuhiko Kinosita Jr., and Masasuke Yoshida
FEBS Lett. 583 (2009) 1121-1126. Abstract PDF (658kB) Journal Supplementary data (465 kB),.

2008

"Neither helix in the coiled coil region of the axle of F1-ATPase plays a significant role in torque production"
Mohammad Delawar Hossain, Shou Furuike, Yasushi Maki, Kengo Adachi, Toshiharu Suzuki, Ayako Kohori, Hiroyasu Itoh, Masasuke Yoshida, and Kazuhiko Kinosita Jr.
Biophys. J. 95 (2008) 4837-4844.     Abstract     PDF (1603 kB)     Journal

"Temperature dependence of the rotation and hydrolysis activities of F1-ATPase"
Shou Furuike, Kengo Adachi, Naoyoshi Sakaki, Rieko Shimo-Kon, Hiroyasu Itoh, Eiro Muneyuki, Masasuke Yoshida, and Kazuhiko Kinosita, Jr.
Biophys. J. 95 (2008) 761-770.     Abstract     PDF (2038 kB)     Journal

"Axle-less F1-ATPase rotates in the correct direction"
Shou Furuike, Mohammad Delawar Hossain, Yasushi Maki, Kengo Adachi, Toshiharu Suzuki, Ayako Kohori, Hiroyasu Itoh, Masasuke Yoshida, and Kazuhiko Kinosita Jr.
Science 319 (2008) 955-958.     Abstract     PDF (4426 kB)     Journal     Supporting Material (955 kB)
    Movie 1 (4069 kB)    Movie 2 (2370 kB)    Movie 3 (1637 kB)    Movie 4 (2100 kB)    Movie 5 (1216 kB)    Movie 6 (1217 kB)

2007

"Coupling of rotation and catalysis in F1-ATPase revealed by single-molecule imaging and manipulation"
Kengo Adachi, Kazuhiro Oiwa, Takayuki Nishizaka, Shou Furuike, Hiroyuki Noji, Hiroyasu Itoh, Masasuke Yoshida, and Kazuhiko Kinosita Jr.
Cell 130 (2007) 309-321.     Abstract     PDF (1917 kB)     Journal

"Myosin V walks by lever action and Brownian motion"
Katsuyuki Shiroguchi, and Kazuhiko Kinosita, Jr.
Science 316 (2007) 1208-1212.     Abstract     PDF (459 kB)     Journal     Supporting Material (3131 kB)
    Movie 1 (1128 kB)     Movie 2 (3094 kB)     Movie 3 (1474 kB)     Movie 4 (985 kB)     Movie 5 (2244 kB)     Movie 6 (794 kB)

"Visualization of RecA filaments and DNA by fluorescence microscopy"
Taro Nishinaka, Yuko Doi, Makiko Hashimoto, Reiko Hara, Takehiko Shibata, Yoshie Harada, Kazuhiko Kinosita, Jr., Hiroyuki Noji, and Eiji Yashima
J. Biochem. 141 (2007) 147–156.     Abstract     PDF (621 kB)     Journal     Supplementary     Suppl.Movie 1     Suppl.Movie2

2006

"The rotor tip inside a bearing of a thermophilic F1-ATPase is dispensable for torque generation"
Mohammad Delawar Hossain, Shou Furuike, Yasushi Maki, Kengo Adachi, M. Yusuf Ali, Mominul Huq, Hiroyasu Itoh, Masasuke Yoshida, and Kazuhiko Kinosita Jr.
Biophys. J., 90 (2006) 4195-4203.     Abstract     PDF (648 kB)     Journal

2005

"Activation of pausing F₁ motor by external force"
Yoko Hirono-Hara, Koji Ishizuka, Kazuhiko Kinosita, Jr., Masasuke Yoshida, and Hiroyuki Noji
Proc. Natl. Acad. Sci. USA, 102 (2005) 4288-4293.      Abstract      PDF (664 kB)      Journal      Supl. Movie (3834 kB)     Movie

"ATP-driven stepwise rotation of F_oF₁-ATP synthase"
Hiroshi Ueno, Toshiharu Suzuki, Kazuhiko Kinosita, Jr., and Masasuke Yoshida
Proc. Natl. Acad. Sci. USA, 102 (2005) 1333-1338.     Abstract     PDF (433 kB)      Journal        Movie

"One rotary mechanism for F₁-ATPase over ATP concentrations from millimolar down to nanomolar"
Naoyoshi Sakaki, Rieko Shimo-Kon, Kengo Adachi, Hiroyasu Itoh, Shou Furuike, Eiro Muneyuki, Masasuke Yoshida, and Kazuhiko Kinosita, Jr.
Biophys. J., 88 (2005) 2047-2056. Abstract PDF (404 kB) Journal Movie

2004

"Unconstrained steps of myosin VI appear longest among known molecular motors"
M. Yusuf Ali, Kazuaki Homma, Atsuko Hikikoshi Iwane, Kengo Adachi, Hiroyasu Itoh, Kazuhiko Kinosita Jr., Toshio Yanagida, and Mitsuo Ikebe
Biophys. J., 86 (2004) 3804-3810.     Abstract     PDF (279 kB)     Journal     Supl. Movie 1     Supl. Movie 2     Movie

"Chemomechanical coupling in F₁-ATPase revealed by simultaneous observation of nucleotide kinetics and rotation"
Takayuki Nishizaka, Kazuhiro Oiwa, Hiroyuki Noji, Shigeki Kimura, Eiro Muneyuki, Masasuke Yoshida, and Kazuhiko Kinosita, Jr.
Nature Struct. Mol. Biol., 11 (2004) 142-148.     Abstract     PDF (1269 kB)     Journal     Supplementary Information     Movie

"Mechanically driven ATP synthesis by F₁-ATPase"
Hiroyasu Itoh, Akira Takahashi, Kengo Adachi, Hiroyuki Noji, Ryohei Yasuda, Masasuke Yoshida, and Kazuhiko Kinosita, Jr.
Nature, 427 (2004) 465-468.     Abstract     PDF (252 kB)     Journal     Supplementary Information    Movie

2003

"Catalysis and rotation of F₁ motor: Cleavage of ATP at the catalytic site occurs in 1 ms before 40° substep rotation"
Katsuya Shimabukuro, Ryohei Yasuda, Eiro Muneyuki, Kiyotaka Y. Hara, Kazuhiko Kinosita, Jr., and Masasuke Yoshida
Proc. Natl. Acad. Sci. USA, 100 (2003) 14731-14736. Abstract PDF (1158 kB) Journal

"The ATP-waiting conformation of rotating F₁-ATPase revealed by single-pair fluorescence resonance energy transfer"
Ryohei Yasuda, Tomoko Masaike, Kengo Adachi, Hiroyuki Noji, Hiroyasu Itoh, and Kazuhiko Kinosita, Jr.
Proc. Natl. Acad. Sci. USA, 100 (2003) 9314-9318. Abstract PDF (891 kB) Journal

2002

"F₁-ATPase changes its conformations upon phosphate release"
Tomoko Masaike, Eiro Muneyuki, Hiroyuki Noji, Kazuhiko Kinosita, Jr., and Masasuke Yoshida
J. Biol. Chem., 277 (2002) 21643-21649. Abstract PDF (417 kB) Journal

"Myosin V is a left-handed spiral motor on the right-handed actin helix"
M. Yusuf Ali, Sotaro Uemura, Kengo Adachi, Hiroyasu Itoh, Kazuhiko Kinosita Jr. and Shin'ichi Ishiwata
Nature Struct. Biol., 9 (2002) 464-467. Abstract PDF (1800 kB) Jounal Movie

2001

"Pause and rotation of F₁-ATPase during catalysis"
Yoko Hirono-Hara, Hiroyuki Noji, Masaya Nishiura, Eiro Muneyuki, Kiyotaka Y. Hara, Ryohei Yasuda, Kazuhiko Kinosita, Jr., and Masasuke Yoshida.
Proc. Natl. Acad. Sci. USA, 98 (2001) 13649-13654. Abstract PDF (781 kB) Journal Movie

"Purine but not pyrimidine nucleotides support rotation of F₁-ATPase"
Hiroyuki Noji, Dirk Bald, Ryohei Yasuda, Hiroyasu Itoh, Masasuke Yoshida, and Kazuhiko Kinosita, Jr.
J. Biol. Chem., 276 (2001) 25480-25486. Abstract PDF (461 kB) Journal

"Resolution of distinct rotational substeps by submillisecond kinetic analysis of F₁-ATPase"
Ryohei Yasuda, Hiroyuki Noji, Masasuke Yoshida, Kazuhiko Kinosita, Jr., and Hiroyasu Itoh
Nature, 410 (2001) 898-904. Abstract PDF (715 kB) Jounal Movie

"Direct observation of DNA rotation during transcription by Escherichia coli RNA polymerase"
Yoshie Harada, Osamu Ohara, Akira Takatsuki, Hiroyasu Itoh, Nobuo Shimamoto, and Kazuhiko Kinosita, Jr.
Nature, 409 (2001) 113-115. Abstract PDF (271 kB) Jounal Movie

2000

"The role of the DELSEED motif of the β subunit in rotation of F₁-ATPase"
Kiyotaka Y. Hara, Hiroyuki Noji, Dirk Bald, Ryohei Yasuda, Kazuhiko Kinosita, Jr., and Masasuke Yoshida
J. Biol. Chem., 275 (2000) 14260-14263. Abstract PDF (170 kB) Jounal

"Characterization of single actomyosin rigor bonds: Load-dependence of lifetime and mechanical properties"
Takayuki Nishizaka, Ryuzo Seo, Hisashi Tadakuma, Kazuhiko Kinosita, Jr., and Shin'ichi Ishiwata
Biophys. J., 79 (2000) 962-974. Abstract PDF (236 kB) Journal Movie

"Stepping rotation of F₁-ATPase visualized through angle-resolved single-fluorophore imaging"
Kengo Adachi, Ryohei Yasuda, Hiroyuki Noji, Hiroyasu Itoh, Yoshie Harada, Masasuke Yoshida, and Kazuhiko Kinosita, Jr.
Proc. Natl. Acad. Sci. USA, 97 (2000) 7243-7247. Abstract PDF (618 kB) Jounal Movie

"Observations of rotation within the F_oF₁-ATP synthase: deciding between rotation of the F_oc subunit ring and artifact"
Satoshi P. Tsunoda, Robert Aggeler, Hiroyuki Noji, Kazuhiko Kinosita, Jr., Masasuke Yoshida, and Roderick A. Capaldi
FEBS Lett., 470 (2000) 244-248. Abstract PDF (139kB) Journal

1999

"Rotation of Escherichia coli F₁-ATPase"
Hiroyuki Noji, Katrin Hasler, Wolfgang Junge, Kazuhiko Kinosita, Jr., Masasuke Yoshida, and Siegfried Engelbrecht.
Biochem. Biophys. Res. Commun., 260 (1999) 597-599. Abstract PDF (85 kB) Journal

"Imaging of thermal activation of actomyosin motors"
Hirokazu Kato, Takayuki Nishizaka, Takashi Iga, Kazuhiko Kinosita, Jr., and Shin'ichi Ishiwata
Proc. Natl. Acad. Sci. USA, 96 (1999) 9602-9606. Abstract PDF (374 kB) Journal

"Tying a molecular knot with optical tweezers"
Yasuharu Arai, Ryohei Yasuda, Ken-ichirou Akashi, Yoshie Harada, Hidetake Miyata, Kazuhiko Kinosita, Jr., and Hiroyasu Itoh
Nature, 399 (1999) 446-448. Abstract PDF (451 kB) Journal Movie

"Protrusive growth from giant liposomes driven by actin polymerization"
Hidetake Miyata, Shuji Nishiyama, Ken-ichirou Akashi, and Kazuhiko Kinosita, Jr.
Proc. Natl. Acad. Sci. USA, 96 (1999) 2048-2053. Abstract PDF (276 kB) Journal Movie

"Single-fluorophore imaging with an unmodified epifluorescence microscope and conventional video camera"
Kengo Adachi, Kazuhiko Kinosita, Jr., and Toshio Ando
J. Microscop., 195 (1999) 125-132. Abstract PDF (196 kB) Journal

1998

"Direct observation of the rotation of ε subunit in F₁-ATPase"
Yasuyuki Kato-Yamada, Hiroyuki Noji, Ryohei Yasuda, Kazuhiko Kinosita, Jr., and Masasuke Yoshida
J. Biol. Chem., 273 (1998) 19375-19377. Abstract PDF (244 kB) Journal

"Synthesis of fluorescent substrates for protein tyrosine phosphatase assays"
Takumi Watanabe, Masaya Imoto, Hiroki Taniguchi, Kazuhiko Kinoshita, Jr., and Kazuo Umezawa
Bioorg. Med. Chem. Lett., 8 (1998) 1301-1302. Abstract PDF (111 kB) Journal

"F₁-ATPase is a highly efficient molecular motor that rotates with discrete 120° steps"
Ryohei Yasuda, Hiroyuki Noji, Kazuhiko Kinosita, Jr., and Masasuke Yoshida
Cell, 93 (1998) 1117-1124. Abstract PDF (4695 kB) Journal Movie

"Formation of giant liposomes promoted by divalent alkali cations: Critical role of electrostatic repulsion"
Ken-ichirou Akashi, Hidetake Miyata, Hiroyasu Itoh, and Kazuhiko Kinosita, Jr.
Biophys. J., 74 (1998) 2973-2982. Abstract PDF (357 kB) Journal

1997

"Modulation of actin filament sliding by mutations of the SH2 cysteine in dictyostelium myosin II"
Yoshikazu Suzuki, Reiko Ohkura, Seiryo Sugiura, Ryohei Yasuda, Kazuhiko Kinoshita, Jr., Masaru Tanokura, and Kazuo Sutoh
Biochem. Biophys. Res. Commun., 234 (1997) 701-706. Abstract PDF (1002 kB) Journal

"Axial rotation of sliding actin filaments revealed by single-fluorophore imaging"
Ichiro Sase, Hidetake Miyata, Shin'ichi Ishiwata, and Kazuhiko Kinosita, Jr.
Proc. Natl. Acad. Sci. USA, 94 (1997) 5646-5650. Abstract PDF (557 kB) Journal Movie

"Direct observation of the rotation of F₁-ATPase"
Hiroyuki Noji, Ryohei Yasuda, Masasuke Yoshida, and Kazuhiko Kinosita, Jr.
Nature, 386 (1997) 299-302. Abstract PDF (1124 kB) Journal Movie

"Cooperative association of actin protomers and crosslinked actin oligomers in filaments at low ionic strength"
Hidetake Miyata, Kazuhiko Kinosita, Jr., and Gerard Marriott
J. Biochem., 121 (1997) 527-533. Abstract PDF (4060 kB) Journal

1996

"Preparation of giant liposomes in physiological conditions and their characterization under an optical microscope"
Ken-ichirou Akashi, Hidetake Miyata, Hiroyasu Itoh, and Kazuhiko Kinosita, Jr.
Biophys. J., 71 (1996) 3242-3250. Abstract PDF (2651 kB) Journal

"Direct measurement of the torsional rigidity of single actin filaments"
Ryohei Yasuda, Hidetake Miyata, and Kazuhiko Kinosita, Jr.
J. Mol. Biol., 263 (1996) 227-236. Abstract PDF (247 kB) Journal Movie

"SEM imaging of single heavy meromyosin molecules on hydrophilic silicon surface"
Taiji Furuno, Hidetake Miyata, Hiroshi Yoshikawa, Kazuhiko Kinosita, Jr., Akira Ikegami, Hiroyuki Sasabe, and Wolfgang Knoll
Bioimages, 4 (1996) 73-78. Abstract PDF (2792 kB) Journal

"Strength and lifetime of the bond between actin and skeletal muscle &alpha-actinin studied with an optical trapping technique"
Hidetake Miyata, Ryohei Yasuda, and Kazuhiko Kinosita, Jr.
Biochim. Biophys. Acta, 1290 (1996) 83-88. Abstract PDF (618 kB) Journal

"Preparation of bead-tailed actin filaments: Estimation of the torque produced by the sliding force in an in vitro motility assay"
Naoya Suzuki, Hidetake Miyata, Shin'ichi Ishiwata, and Kazuhiko Kinosita, Jr.
Biophys. J., 70 (1996) 401-408. Abstract PDF (2557 kB) Journal

1995

"Regulatory mechanisms of the acrosome reaction revealed by multiview microscopy of single starfish sperm"
Ichiro Sase, Tatsuyuki Okinaga, Motonori Hoshi, Gerald W. Feigenson, and Kazuhiko Kinosita, Jr.
J. Cell Biol., 131 (1995) 963-973. Abstract PDF (3976 kB) Journal Movie

"Unbinding force of a single motor molecule of muscle measured using optical tweezers"
Takayuki Nishizaka, Hidetake Miyata, Hiroshi Yoshikawa, Shin'ichi Ishiwata, and Kazuhiko Kinosita, Jr.
Nature, 377 (1995) 251-254. Abstract PDF (908 kB) Journal Movie

"Realtime imaging of single fluorophores on moving actin with an epifluorescence microscope"
Ichiro Sase, Hidetake Miyata, John E. T. Corrie, James S. Craik, and Kazuhiko Kinosita, Jr.
Biophys. J., 69 (1995) 323-328. Abstract PDF (1808 kB) Journal

"Mechanical measurements of single actomyosin motor force"
Hidetake Miyata, Hiroshi Yoshikawa, Hiroyuki Hakozaki, Naoya Suzuki, Taiji Furuno, Akira Ikegami, Kazuhiko Kinosita, Jr., Takayuki Nishizaka, and Shin'ichi Ishiwata
Biophys. J., 68 (1995) 286s-290s. Abstract PDF (770 kB) Journal

"Spatiotemporal relationships among early events of fertilization in sea urchin eggs revealed by multiview microscopy"
Keisuke Suzuki, Yuriko Tanaka, Yoko Nakajima, Ken'ichi Hirano, Hiroyasu Itoh, Hidetake Miyata, Tsuyoshi Hayakawa, and Kazuhiko Kinosita, Jr.
Biophys. J., 68 (1995) 739-748. Abstract PDF (2864 kB) Journal Movie

1994

"Transformation of actin-encapsulating liposomes induced by cytochalasin D"
Hidetake Miyata, and Kazuhiko Kinosita, Jr.
Biophys. J., 67 (1994) 922-928. Abstract PDF (1724 kB) Journal

"Stepwise motion of an actin filament over a small number of heavy meromyosin in in vitro motility assay"
Hidetake Miyata, Hiroyuki Hakozaki, Hiroshi Yoshikawa, Naoya Suzuki, Kazuhiko Kinosita, Jr., Takayuki Nishizaka, and Shin'ichi Ishiwata
J. Biochem. (Tokyo), 115 (1994) 644-647. Abstract PDF (1568 kB) Journal

1993

"Time courses of cell electroporation as revealed by submicrosecond imaging of transmembrane potential"
Masahiro Hibino, Hiroyasu Itoh, and Kazuhiko Kinosita, Jr.
Biophys. J., 64 (1993) 1789-1800. Abstract PDF (2632 kB) Journal

"Steady-state deformation of a vesicle in alternating electric fields"
Hiroyuki Hyuga, Kazuhiko Kinosita, Jr., and Nobuyoshi Wakabayashi
Bioelectrochem. Bioenerg., 32 (1993) 15-25. Abstract PDF (675kB) Journal

1992

"Photomodulation of the nucleating activity of a photocleavable crosslinked actin dimer"
Gerard Marriott, Hidetake Miyata, and Kazuhiko Kinosita, Jr.
Biochem. Intern., 26 (1992) 943-951. Abstract PDF (2206 kB) Journal

"Fluorescence quenching measurements of the membrane bound lipid haptens with different length spacers"
Kazuko Kimura, Yoji Arata, Tatsuji Yasuda, Kazuhiko Kinosita, Jr., and Mamoru Nakanishi
Biochim. Biophys. Acta, 1104 (1992) 9-14. Abstract PDF (375 kB) Journal

1991

"Transient and steady-state deformations of a vesicle with an insulating membrane in response to step-function and alternating electric fields"
Hiroyuki Hyuga, Kazuhiko Kinosita, Jr., and Nobuyoshi Wakabayashi
Jpn. J. Appl. Phys., 30 (1991) 2649-2656. Abstract PDF (1289kB) Journal

"Dual-view microscopy with a single camera: Real-time imaging of molecular orientations and calcium"
Kazuhiko Kinosita, Jr., Hiroyasu Itoh, Shin'ichi Ishiwata, Ken'ichi Hirano, Takayuki Nishizaka, and Tsuyoshi Hayakawa
J. Cell Biol., 115 (1991) 67-73. Abstract PDF (1880 kB) Journal Movie

"Deformation of vesicles under the influence of strong electric fields II"
Hiroyuki Hyuga, Kazuhiko Kinosita, Jr., and Nobuyoshi Wakabayashi
Jpn. J. Appl. Phys., 30 (1991) 1333-1335. Abstract PDF (448 kB) Journal

"Deformation of vesicles under the influence of strong electric fields"
Hiroyuki Hyuga, Kazuhiko Kinosita, Jr., and Nobuyoshi Wakabayashi
Jpn. J. Appl. Phys., 30 (1991) 1141-1148. Abstract PDF (1108 kB) Journal

"Membrane conductance of an electroporated cell analyzed by submicrosecond imaging of transmembrane potential"
Masahiro Hibino, Masaya Shigemori, Hiroyasu Itoh, Kuniaki Nagayama, and Kazuhiko Kinosita, Jr.
Biophys. J., 59 (1991) 209-220. Abstract PDF (2776 kB) Journal

1990

"Location of membrane-bound hapten with different length spacers"
Kazuko Kimura, Yoji Arata, Tatsuji Yasuda, Kazuhiko Kinosita, Jr., and Mamoru Nakanishi
Immunol., 69 (1990) 323-328. Abstract PDF (2203 kB) Journal

1989

"Independent flexible motion of submolecular domains of the Ca²⁺,Mg²⁺ATPase of sarcoplasmic reticulum measured by time-resolved fluorescence depolarization of site-specifically attached probes"
Sei-ichi Suzuki, Suguru Kawato, Tsutomu Kouyama, Kazuhiko Kinosita, Jr., Akira Ikegami, and Masao Kawakita
Biochemistry, 28 (1989) 7734-7740. Abstract PDF (829kB) Journal

"Correlation between internal motion and emission kinetics of tryptophan residues in proteins"
Tsutomu Kouyama, Kazuhiko Kinosita, Jr., and Akira Ikegami
Eur. J. Biochem., 182 (1989) 517-521. Abstract PDF (1575 kB) Journal

1988

"Dynamic structure of F-actin and its Ca-dependent regulation under the influence of tropomyosin-troponin system"
Koshin Mihashi, Atsushi Ooi, Naoya Suzuki, Hideyuki Yoshimura, and Kazuhiko Kinosita, Jr.
Mol. Cell. Biol. (Life Sci. Adv.), 7 (1988), 143-148. Abstract PDF (1900 kB) Journal

"Chromophore of bacteriorhodopsin is closer to the cytoplasmic surface of purple membrane. Fluorescence energy transfer on oriented membrane sheets"
Jun Otomo, Akihiro Tomioka, Kazuhiko Kinosita, Jr., Hideyuki Miyata, Yuko Takenaka, Tsutomu Kouyama, and Akira Ikegami
Biophys. J., 54 (1988) 57-64. Abstract PDF (2398 kB) Journal

"Electroporation of cell membrane visualized under a pulsed-laser fluorescence microscope"
Kazuhiko Kinosita, Jr., Ikuo Ashikawa, Nobuyuki Saita, Hideyuki Yoshimura, Hiroyasu Itoh, Kuniaki Nagayama, and Akira Ikegami
Biophys. J., 53 (1988) 1015-1019. Abstract PDF (1814 kB) Journal

1987

"Transmembrane location of retinal in purple membrane. Fluorescence energy transfer in maximally packed donor-acceptor systems"
Tadaatsu Kometani, Kazuhiko Kinosita, Jr., Taiji Furuno, Tsutomu Kouyama, and Akira Ikegami
Biophys. J., 52 (1987) 509-517. Abstract PDF (1916 kB) Journal

"Changes of the DNA packaging mode during boar sperm maturation"
Ikuo Ashikawa, Kazuhiko Kinosita, Jr., Akira Ikegami, and Tohru Tobita
Biochim. Biophys. Acta, 908 (1987) 263-267. Abstract PDF (386kB) Journal

"Rotational motions of myosin heads in myofibril studied by phosphorescence anisotropy decay measurements"
Shin'ichi Ishiwata, Kazuhiko Kinosita, Jr., Hideyuki Yoshimura, and Akira Ikegami
J. Biol. Chem., 262 (1987) 8314-8317. Abstract PDF (1758 kB) Journal

"Criteria for the rapid diffusion limit of fluorescence energy transfer"
Kazuhiko Kinosita, Jr., Tsutomu Kouyama, and Akira Ikegami
J. Chem. Phys., 86 (1987) 3273-3282. Abstract PDF (1173 kB) Journal

1986

"Low-temperature laser photolysis of benzophenone in neat diethylaniline: Optical detection of an excited triplet state with a strong charge-transfer structure at 77K"
Mikio Hoshino, Hiroshi Seki, Masao Kaneko, Kazuhiko Kinoshita, Jr., and Haruo Shizuka
Chem. Phys. Lett., 132 (1986) 209-212. Abstract PDF (363kB) Journal

"Environmental modulation of C-terminus dynamic structure in bacteriorhodopsin"
Jeffrey Marque, Kazuhiko Kinosita, Jr., Rajini Govindjee, Akira Ikegami, Thomas G. Ebrey, and Jun Otomo
Biochemistry, 25 (1986) 5555-5559. Abstract PDF (672 kB) Journal

1985

"Interaction between Ca²⁺ and dipalmitoylphosphatidylcholine membranes. II. Fluorescence anisotropy study"
Ryoichi Kataoka, Shuji Aruga, Shigeki Mitaku, Kazuhiko Kinosita, Jr., and Akira Ikegami
Biophys. Chem., 21 (1985) 277-284. Abstract PDF (581kB) Journal

"Increased stability of the higher-order structure of chicken erythrocyte chromatin. Nanosecond anisotropy studies of intercalated ethidium"
Ikuo Ashikawa, Kazuhiko Kinosita, Jr., Akira Ikegami, Yoshifumi Nishimura, and Masamichi Tsuboi
Biochemistry, 24 (1985) 1291-1297. Abstract PDF (1749 kB) Journal

"Reversible and irreversible modification of erythrocyte-membrane permeability by electric field"
Engin H. Serpersu, Kazuhiko Kinosita, Jr., and Tian Yow Tsong
Biochim. Biophys. Acta, 812 (1985) 779-785. Abstract PDF (542kB) Journal

"The excited-state dynamics of bacteriorhodopsin"
Tsutomu Kouyama, Kazuhiko Kinosita, Jr., and Akira Ikegami
Biophys. J., 47 (1985) 43-54. Abstract PDF (2288 kB) Journal

1984

"Submicrosecond and microsecond rotational motions of myosin head in solution and in myosin synthetic filaments as revealed by time-resolved optical anisotropy decay measurements"
Kazuhiko Kinosita, Jr., Shin'ichi Ishiwata, Hideyuki Yoshimura, Hiroshi Asai, and Akira Ikegami
Biochemistry, 23 (1984) 5963-5975. Abstract PDF (3403 kB) Journal

"Torsional motion of eosin-labeled F-actin as detected in the time-resolved anisotropy decay of the probe in the sub-millisecond time range"
Hideyuki Yoshimura, Koshin Mihashi, Takuhiro Nishio, Kazuhiko Kinosita, Jr., and Akira Ikegami
J. Mol. Biol., 179 (1984) 453-467. Abstract PDF (2977 kB) Journal

"Internal motion of DNA in bacteriophages"
Ikuo Ashikawa, Taiji Furuno, Kazuhiko Kinosita, Jr., Akira Ikegami, Hideo Takahashi, and Hideo Akutsu
J. Biol. Chem., 259 (1984) 8338-8344. Abstract PDF (1258 kB) Journal

"Rotational dynamics of immunoglobulins with fluorescent haptens on a membrane surface"
Hiroshi Osada, Mamoru Nakanishi, Masamichi Tsuboi, Kazuhiko Kinosita, Jr., and Akira Ikegami
Biochim. Biophys. Acta, 773 (1984) 321-324. Abstract PDF (264kB) Journal

"The dynamics of lipid motion in sarcoplasmic reticulum membranes determined by steady-state and time-resolved fluorescence measurements on 1,6-diphenyl1,3,5-hexatriene and related molecules"
Christopher D. Stubbs, Kazuhiko Kinosita, Jr., Felix Munkonge, Peter J. Quinn, and Akira Ikegami
Biochim. Biophys. Acta, 775 (1984) 374-380. Abstract PDF (518kB) Journal

"Dynamics of Z-form DNA"
Ikuo Ashikawa, Kazuhiko Kinosita, Jr., and Akira Ikegami
Biochim. Biophys. Acta, 782 (1984) 87-93. Abstract PDF (480kB) Journal

"Reevaluation of the wobbling dynamics of diphenylhexatriene in phosphatidylcholine and cholesterol/ phosphatidylcholine membranes"
Kazuhiko Kinosita, Jr., and Akira Ikegami
Biochim. Biophys. Acta, 768 (1984) 521-527. Abstract PDF (375jB) Journal

1983

"Internal motion of deoxyribonucleic acid in chromatin. Nanosecond fluorescence studies of intercalated ethidium"
Ikuo Ashikawa, Kazuhiko Kinosita, Jr., Akira Ikegami, Yoshifumi Nishimura, Masamichi Tsuboi, Kazutada Watanabe, Koujiro Iso, and Takashi Nakano
Biochemistry 22 (1983) 6018-6026. Abstract PDF (1161 kB) Journal

"Possible association of NADPH-cytochrome P-450 reductase and cytochrome P-450 in reconstituted phospholipid vesicles"
Yukio Nisimoto, Kazuhiko Kinosita, Jr., Akira Ikegami, Norio Kawai, Ichiro Ichihara, and Yukio Shibata
Biochemistry 22 (1983) 3586-3594. Abstract PDF (2183 kB) Journal

"Fluorescence energy transfer studies of transmembrane location of retinal in purple membrane"
Tsutomu Kouyama, Kazuhiko Kinosita, Jr., and Akira Ikegami
J. Mol. Biol. 165 (1983) 91-107. Abstract PDF (3147 kB) Journal

"Internal motion of F-actin in 10^-6-10^-3 sec time range studied by transient absorption anisotropy; Detection of torsional motion"
Koshin Mihashi, Hideyuki Yoshimura, Takuhiro Nishio, Akira Ikegami, and Kazuhiko Kinosita, Jr.
J. Biochem. 93 (1983) 1705-1707. Abstract PDF (655 kB) Journal

"Dynamics of DNA in chromatin and DNA binding mode to core protein"
Ikuo Ashikawa, Kazuhiko Kinosita, Jr., Akira Ikegami, Yoshifumi Nishimura, Masamichi Tsuboi, Kazutada Watanabe, and Koujiro Iso
J. Biochem. 93 (1983) 665-668. Abstract PDF (751 kB) Journal

1982

"Nanosecond fluorometric investigation of hydrodynamic properties of adenosine triphosphatase from thermophilic bacterium PS3"
Kazuhiko Kinosita, Jr., Akira Ikegami, Masasuke Yoshida, and Yasuo Kagawa
J. Biochem. 92 (1982) 2043-2046. Abstract PDF (2076 kB) Journal

"Photoinduced electron transfer from polystyrene pendant tris(2,2'bipyridyl)ruthenium(II) complex to ethylviologen"
Masao Kaneko, Masahisa Ochiai, Kazuhiko Kinosita, Jr., and Akira Yamada
J. Polym. Sci. 20 (1982) 1011-1019. Abstract PDF (427kB) Journal

"On the wobbling-in-cone analysis of fluorescence anisotropy decay"
Kazuhiko Kinosita, Jr., Akira Ikegami, and Suguru Kawato
Biophys. J. 37 (1982) 461-464. Abstract PDF (683 kB) Journal

1981

"Location and orientation of the chromophore in bacteriorhodopsin. Analysis by fluorescence energy transfer"
Tsutomu Kouyama, Yoshiaki Kimura, Kazuhiko Kinosita, Jr., and Akira Ikegami
J. Mol. Biol. 153 (1981) 337-359. Abstract PDF (7432 kB) Journal

"Time-dependent absorption anisotropy and rotational diffusion of proteins in membranes"
Suguru Kawato, and Kazuhiko Kinosita, Jr.
Biophys. J. 36 (1981) 277-296. Abstract PDF (1850 kB) Journal

"The effect of cytochrome oxidase on lipid chain dynamics: A nanosecond fluorescence depolarization study"
Kazuhiko Kinosita, Jr., Suguru Kawato, Akira Ikegami, Satoshi Yoshida, and Yutaka Orii
Biochim. Biophys. Acta 647 (1981) 7-17. Abstract PDF (873kB) Journal

"Dynamic structure of biological membranes as probed by 1,6-diphenyl-1,3,5-hexatriene: A nanosecond fluorescence depolarization study"
Kazuhiko Kinosita, Jr., Ryoichi Kataoka, Yoshiaki Kimura, Osamu Gotoh, and Akira Ikegami
Biochemistry 20 (1981) 4270-4277. Abstract PDF (1086 kB) Journal

"Effect of double bonds on the dynamic properties of the hydrocarbon region of lecithin bilayers"
Christopher D. Stubbs, Tsutomu Kouyama, Kazuhiko Kinosita, Jr., and Akira Ikegami
Biochemistry 20 (1981) 4257-4262. Abstract PDF (788 kB) Journal

"Immobility of the chromophore in bacteriorhodopsin"
Tsutomu Kouyama, Yoshiaki Kimura, Kazuhiko Kinosita, Jr., and Akira Ikegami
FEBS Lett. 124 (1981) 100-104. Abstract PDF (396 kB) Journal

"Nanosecond time-resolved fluorescence investigations of temperature-induced conformational changes in cytochrome oxidase in phosphatidylcholine vesicles and solubilized systems"
Suguru Kawato, Satoshi Yoshida, Yutaka Orii, Akira Ikegami, and Kazuhiko Kinosita, Jr.
Biochim. Biophys. Acta 634 (1981) 85-92. Abstract PDF (431kB) Journal

1979

"Voltage-induced conductance in human erythrocyte membranes"
Kazuhiko Kinosita, Jr., and Tian Yow Tsong
Biochim. Biophys. Acta 554 (1979) 479-497. Abstract PDF (1114kB) Journal

1978

"Survival of sucrose-loaded erythrocytes in the circulation"
Kazuhiko Kinosita, Jr., and Tian Yow Tsong
Nature 272 (1978) 258-260. Abstract PDF (1355kB) Journal

"Effect of cholesterol on the molecular motion in the hydrocarbon region of lecithin bilayers studied by nanosecond fluorescence techniques"
Suguru Kawato, Kazuhiko Kinosita, Jr., and Akira Ikegami
Biochemistry 17 (1978) 5026-5031. Abstract PDF (712 kB) Journal

1977

"A theory of fluorescence polarization decay in membranes"
Kazuhiko Kinosita, Jr., Suguru Kawato, and Akira Ikegami
Biophys. J. 20 (1977) 289-305. Abstract PDF (1525kB) Journal

"Voltage-induced pore formation and hemolysis of human erythrocytes"
Kazuhiko Kinosita, Jr., and Tian Yow Tsong
Biochim. Biophys. Acta 471 (1977) 227-242. Abstract PDF (982kB) Journal

"Formation and resealing of pores of controlled sizes in human erythrocyte membrane"
Kazuhiko Kinosita, Jr., and Tian Yow Tsong
Nature 268 (1977) 438-441. Abstract PDF (1554kB) Journal

"Hemolysis of human erythrocytes by a transient electric field"
Kazuhiko Kinosita, Jr., and Tian Yow Tsong
Proc. Natl. Acad. Sci. U. S. 74 (1977) 1923-1927. Abstract PDF (1021 kB) Journal

"Dynamic structure of lipid bilayers studied by nanosecond fluorescence techniques"
Suguru Kawato, Kazuhiko Kinosita, Jr., and Akira Ikegami
Biochemistry 16 (1977) 2319-2324. Abstract PDF (662 kB) Journal

1976

"Construction of a nanosecond fluorometric system for applications to biological samples at cell or tissue levels"
Kazuhiko Kinosita, Jr., Shigeki Mitaku, Akira Ikegami, Nobuo Ohbo, and Tosiyasu L. Kunii
Jpn. J. Appl. Phys. 15 (1976) 2433-2440. Abstract PDF (2701 kB) Journal

1975

"Degree of dissociation of apohemoglobin studied by nanosecond fluorescence polarization technique"
Kazuhiko Kinosita, Jr., Shigeki Mitaku, and Akira Ikegami
Biochim. Biophys. Acta 393 (1975) 10-14. Abstract PDF (278kB) Journal

Top